CGSC Strain#: 6196
Strain Designation: W1485D      Source of Strain: N. Davidson
Sex: F+ Chromosomal Markers: γδ(3X)=::Tn1000, λ^-, rph-1
Strain Comments:

• The strain contains 3 insertions of Tn1000 (gamma-delta).
• We suggest calling this strain W1485D in order to distinguish it from the parent strain, which has no insertions of Tn1000.
• γδ(3X)=::Tn1000-- : The strain carries three insertions of Tn1000 (γδ).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• γδ(3X)=::Tn1000 was formerly called gamma delta 3X

References:

• Guyer, M.S., R.E. Reed, T. Steitz, K.B. Low 1981. Identification of a sex-factor-affinity site in E. coli as gamma delta. Cold Spr.Harb.Symp.Quant.Biol. 45:135-140