CGSC Strain#: 6102
Strain Designation: SK472      Source of Strain: S.R. Kushner
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, thiE1, opgB202::Tn10, serB22
Strain Comments:

• Grows poorly on serine.
• This strain may no longer be Hfr.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• opgB202::Tn10-- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
• serB22-- : strain grows poorly on serine,
• opgB202::Tn10 was formerly called
• thiE1 was formerly called thi-1
• = tetracycline resistant

References: