CGSC Strain#: 6100
Strain Designation: JC10241      Source of Strain: A.J. Clark
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-300, λ^-, srlD300::Tn10, relA1, rpsE2300(SpcR), spoT1, ilv-318, thiE1
Strain Comments:

• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• srlD300::Tn10 was formerly called srlC300::Tn10
• thiE1 was formerly called thi-1
• = tetracycline resistant
• SpcR = spectinomycin resistant

References:

• Csonka, LN, AJ Clark 1980. Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains. J. Bacteriol. 143(1):529-30.