CGSC Strain#: 6085
Strain Designation: HMS85      Source of Strain: C.C. Richardson
Sex: F- Chromosomal Markers: polB100, leu-85, lacZ53(Am), λ^-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), deoC2, lys-34
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Campbell, JL, L Soll, CC Richardson 1972. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc. Natl. Acad. Sci. U.S.A. 69(8):2090-4.
• Campbell, JL, H Shizuya, CC Richardson 1974. Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12. J. Bacteriol. 119(2):494-9.