CGSC Strain#: 6060
Strain Designation: CS520      Source of Strain: J. Carbon
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: tyrT58(AS), trpA58, relA1, spoT1, metB1, glyV-50
Strain Comments:

• This is the strain from which the DNA of the Clarke and Carbon plasmid bank was made.
• This strain is probably Rac^- also. See: Kaiser and Murray 1979 Mol.Gen.Genet. 175:159. glyV50 was called suA58 or A58sul. See: Guest and Yanofsky 1965 J.Mol.Biol. 12:793.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• tyrT58(AS) was formerly called supF58
• AS = amber(UAG) suppressor

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