CGSC Strain#: 6027
Strain Designation: CA8306      Source of Strain: J.R. Beckwith
Other Designations: EM1328
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, ΔcyaA854, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ΔcyaA854-- is a 200 bp frameshift deletion that generates a new translation start (Glaser et al., below)
• thiE1 was formerly called thi-1

References:

• Brickman, E., L. Soll, J.R. Beckwith 1973. Genetic characterization of mutations which affect catabolite-sensitive operons in Escherichia coli, including deletions of the gene for adenyl cyclase. J.Bacteriol. 116:582-587
• Kumar, A., V.F. Maples, W.S. Champney 1976. Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli. J.Bacteriol. 125:545-555
• Glaser, P, A Roy, A Danchin 1989. Molecular characterization of two cya mutations, cya-854 and cyaR1. J. Bacteriol. 171(9):5176-8.