CGSC Strain#: 5859
Strain Designation: JRG548      Source of Strain: J.R. Guest
Other Designations: WGASsdh-9
Sex: F- Chromosomal Markers: sdh-9(Am), gal-25, λ^-, trpA9761(Am), rpsL231(strR), iclR7(Const), trpR72(Am), chl-7
Strain Comments:

• sdh-9(Am)-- is an amber mutation.
• iclR7(Const)-- renders this strain constitutive for isocitrate lyase.
• trpA9761(Am) was formerly called trp-40
• trpA9761(Am) was formerly called trp-47
• Am = amber(UAG) mutation
• Const = constitutive
• strR = streptomycin resist.

References:

• Creaghan, IT, JR Guest 1972. Amber mutants of the -ketoglutarate dehydrogenase gene of Escherichia coli K12. J. Gen. Microbiol. 71(2):207-20.