CGSC Strain#: 7712
Strain Designation: TN3151      Source of Strain: T.Nystrom and A.Farewell
Sex: F- Chromosomal Markers: λ^-, IN(rrnD-rrnE)1, uspA1::kan, rph-1
Strain Comments:

• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• uspA1::kan-- lags on fresh med. see comment uspA gene.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Nystrom, T., F.C. Neidhardt 1993. Isolation and properties of a mutant of Escherichia coli with an insertional activation of the uspA gene, which encodes a universal stress protein. J.Bacteriol. 175:3949-3956
• Farewell, A., A.A. Diez, C.C. DiRusso, T. Nystrom 1996. Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes. J.Bacteriol. 178:6443-6450