CGSC Strain#: 5523
Strain Designation: DF220      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ompF627(T2R), gapA1(Unst), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• This strain grows very poorly on glycerol plus malate unless the medium is supplemented with casamino acids. The gap-1 marker reverts at a relatively high rate.
• gapA1(Unst)-- reverts at a relatively high rate.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant
• Unst = unstable

References:

• Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.