CGSC Strain#: 7708
Strain Designation: AW804      Source of Strain: J. Adler
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), fhuA31, lacY1, tsx-78, hemH230, gsk-3055::Tn10, glnX44(AS), galK2(Oc), galT22, λ^-, hisG4(Oc), rfbC1, mgl-51, rpsL136(strR), xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• hemH230-- results in 100-fold greater accumulation of non-iron porphyrins and 100-fold greater sensitivity in tumbling to blue light than Hem+ parent.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gsk-3055::Tn10 was formerly called zba-3055::Tn10
• hemH230 was formerly called hemH1
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Yang, H., H. Inokuchi, J. Adler 1995. Phototaxis away from blue light by an Escherichia coli mutant accumulating protoporphyrin IX. Proc.Natl.Acad.Sci.USA 92:7332-7336
• Yang, H., A. Sasarman, H. Inokuchi, J. Adler 1996. Non-iron porphyrins cause tumbling to blue light by an Escherichia coli mutant defective in hemG. Proc.Natl.Acad.Sci.USA 93:2459-2463