CGSC Strain#: 5688
Strain Designation: JRG596      Source of Strain: J.R. Guest
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: secA214(aziR), Δ(aroP-aceF)73, fhuA54, tsx-97, tonB54, pps-1, relA1, thyA56, spoT1, metB1
Strain Comments:

• This strain is apparently no longer Hfr. This strain was formerly called K DE15.
• thyA56-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• secA214(aziR) was formerly called secA14
• secA214(aziR) was formerly called azi-14
• aziR = azide resistance

References:

• Langley, D., J.R. Guest 1977. Biochemical studies of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. J.Gen.Microbiol. 99:263-276