CGSC Strain#: 7524
Strain Designation: β2073      Source of Strain: D. Mazel
Other Designations: Beta2073
Other Designations: B2073
Sex: F- Chromosomal Markers: λ^-, ΔpabB7::kan, Δ(def-fmt)871(::cat,del-ins), rph-1, lamB2000(λ^R)
Strain Comments:

• The strain is slow growing and temperature sensitive as well as Mal^-, λ-resistant, paba^-, and kanamycin and chloramphenicol resistant.
• ΔpabB7::kan-- Allele from BN600 of B.Nichols
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• lamB2000(λ^R)-- spontaneous mutation in β2073
• = kanamycin resistant
• = chloramphenicol resistant
• cat = chloramphenicol acetyl transferase
• del-ins = deletion-insertion
• LamR = Lambda-resistant

References:

• Mazel, D., S. Pochet, P. Marliere 1994. Genetic characterization of polypeptide deformylase, a distinctive enzyme of eubacterial translation. EMBO J. 13:914-923