CGSC Strain#: 5675
Strain Designation: C600-T44      Source of Strain: Goldthwaite & Jacob
Other Designations: C600 44 GT19
Other Designations: T44
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, e14-, recA441(ts), glpR200(glp^c), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA441(ts)-- formerly tif-1 (temp.-induced filamentation). Not a Rec^- phenotype, "constitutive protease activity" not requiring ss-DNA binding at hi temp.
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• ts = Temperature Sensitive

References:

• Goldthwaite, F. Jacob 1964. Compt.Rend.Acad.Sci.Paris 259:661-0
• Castellazzi, M, J George, G Buttin 1972. Prophage induction and cell division in E. coli. I. Further characterization of the thermosensitive mutation tif-1 whose expression mimics the effect of UV irradiation. Mol. Gen. Genet. 119(2):139-52.