CGSC Strain#: 5534
Strain Designation: JRG361      Source of Strain: J.R. Guest
Other Designations: Hlpd10
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: secA207(aziR), lpd-10, λ^-, relA1, spoT1, thiE1
Strain Comments:

• This strain is less vigorous than JRG301 (CGSC No. 5518), another lpd mutant
• This strain was formerly called H lpd 10.
• lpd-10-- : add glucose to LB or other complete media; add 2 mM acetate and 2 mM succinate to glucose minimal media also. Glucose-free media will select against the mutation
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• secA207(aziR) was formerly called azi-7
• thiE1 was formerly called thi-1
• aziR = azide resistance

References:

• Guest, JR, IT Creaghan 1973. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants. J. Gen. Microbiol. 75(1):197-210.