CGSC Strain#: 7661
Strain Designation: BW26437      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(lacA-lacZ)514(::kan), λ^-, rph-1
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.