CGSC Strain#: 7659
Strain Designation: BW26438      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(araD-araB)567, Δ(lacA-lacZ)515(::cat), λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514
Strain Comments:

• Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(araD-araB)567 was formerly called Δ araBAD_AH33
• Δ(lacA-lacZ)515(::cat) was formerly called DE(lacZYA)514::cat
• Δ(rhaD-rhaB)568 was formerly called Δ rhaBAD_LD78
• = chloramphenicol resistant
• cat = chloramphenicol acetyl transferase

References:

• Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.