CGSC Strain#: 7598
Strain Designation: CY265      Source of Strain: J.E. Cronan
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: Δ(aroP-aceF)73, lacZ608(Am), λ^-
Strain Comments:

• This strain is grown on Luria plus acetate.
• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• Am = amber(UAG) mutation

References:

• Chang, Y.-Y., J.E. Cronan 1982. Mapping nonselectable genes of Escherichia coli by using transposon Tn10 location of a gene affecting pyruvate oxidase. J.Bacteriol. 151:1279-1289
• Chang, Y.-Y., J.E. Cronan 1982. Mapping nonselectable genes of Escherichia coli by using transposon Tn10 location of a gene affecting pyruvate oxidase. J.Bacteriol. 151:1279-1289