CGSC Strain#: 5682
Strain Designation: Lin201      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, ompF627(T2R), glpA25, fadL701(T2R), relA1, glpD10, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• glpD10-- : Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• glpD10-- : Loss also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Kistler, WS, CA Hirsch, NR Cozzarelli, EC Lin 1969. Second pyridine nucleotide-independent 1-alpha-glycerophosphate dehydrogenase in Escherichia coli K-12. J. Bacteriol. 100(2):1133-5.
• Kistler, WS, EC Lin 1971. Anaerobic L- -glycerophosphate dehydrogenase of Escherichia coli: its genetic locus and its physiological role. J. Bacteriol. 108(3):1224-34.