CGSC Strain#: 5573
Strain Designation: RH041      Source of Strain: R.B. Helling
Other Designations: AB259 6-1
Other Designations: RBH041
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, purB67, e14-, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Adams, B.S., R.B. Helling 1970. Nalidixic acid-resistant auxotrophs of Escherichia coli. J.Bacteriol. 104:1027-1030
• Curtis, S.J., W. Epstein 1975. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. J.Bacteriol. 122:1189-1199