CGSC Strain#: 5553
Strain Designation: GE384      Source of Strain:
Sex: F' Episome/Plasmid: F141
   Plasmid Markers/Mutations:
Chromosomal Markers: leuB6(Am), lac-290, phoA1, tsx-96, recA1, aroE24, rpsL186(strR), cysG132, malT1(λ^R), ilv-591, gal-290, spc-18
Strain Comments:

• This strain should be grown on medium containing ilv and leu, and lacking cys and aromatic amino acids.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• Am = amber(UAG) mutation
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Andresson, O.S., R.A. Magnusdottir, G. Eggertsson 1976. Deletions of ribosomal protein genes in Escherichia coli merodiploids heterozygous for resistance to streptomycin and spectinomycin. Mol.Gen.Genet. 144:127-130