CGSC Strain#: 5199
Strain Designation: DG76      Source of Strain: D. Glaser
Sex: F- Chromosomal Markers: leuB6(Am), λ^-, thyA47, rpsL153(strR), deoC3
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Wolf, B, A Newman, DA Glaser 1968. On the origin and direction of replication of the Escherichia coli K12 chromosome. J. Mol. Biol. 32(3):611-29.