CGSC Strain#: 5514
Strain Designation: Lin 135      Source of Strain: E.C.C. Lin
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), secA208, fhuA2::IS2, lacY1, glnX44(AS), sdh-3, λ^-, rpsL135(strR), glpR2(glp^c), glpD3, xyl-7, mtlA2, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• secA208-- : azi is now secA
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• secA208 was formerly called azi-8
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• glp^c = constitutive expr.glp regulon
• strR = streptomycin resist.

References:

• Miki, K, EC Lin 1973. Enzyme complex which couples glycerol-3-phosphate dehydrogenation to fumarate reduction in Escherichia coli. J. Bacteriol. 114(2):767-71.