CGSC Strain#: 7562
Strain Designation: DS111      Source of Strain: S. Rahuel-Clermont
Sex: F- Chromosomal Markers: λ^-, Δepd-11::Ery, rph-1
Strain Comments:

• These gap/epd-deleted strains should be grown on minimal supplementd with casamino acids, tryptophan, Met, Thi, succinate and glycerol plus antibiotic. They will not grow on LB or glucose media.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δepd-11::Ery was formerly called gapB11::Erm

References:

• Seta, F.D., S. Boschi-Muller, M.L. Vignais, G. Branlant 1997. Characterization of Escherichia coli strains with gapA and gapB genes deleted. J.Bacteriol. 179:5218-5221