CGSC Strain#: 5089
Strain Designation: Lin 402      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, ompF627(T2R), glpA9, fadL701(T2R), relA1, Δ(malQ-glpR)14(glp^c), pitA10, spoT1
Strain Comments:

• glpA9-- Loss of GlpA or D also results in stasis with gluconeogenic C sources, e.g., succinate. glpA or glpD mutants should be grown on Min.salts+ 1% glycerol-free casein hydrolysate + 1%glucose
• glpA9-- Loss of anaerobic G-3-P deHase results in inability of strains to grow on glycerol or glycerol-3-P as sole C-source under anaerobic conditions.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(malQ-glpR)14(glp^c) was formerly called glpR5
• Δ(malQ-glpR)14(glp^c) was formerly called malA34
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Freedberg, W.B., E.C.C. Lin 1973. Three kinds of controls affecting the expression of the glp regulon in Escherichia coli. J.Bacteriol. 115:816-816