CGSC Strain#: 5087
Strain Designation: H560      Source of Strain: H. Hoffmann-Berling
Sex: F+ Chromosomal Markers: tsx-79, endA2, rpsL8 or rpsL154(strR), polA1(Am)
Strain Comments:

• 1. We have received reports that H560 is not a very satisfactory strain. It is said to have received around 1/2 of the chromosome from E. coli strain C. It is F+ and is sensitive to acridine
• 2.orange so that it is difficult to cure. It is temperature-sensitive at 45 degrees C. The strain is rather difficult to transduce. It forms "odd" colonies on plates and tends to die out easily.
• 3. It seems likely that there is a Thy^- derivative of H560 which is often called simply ``H560". We suggest calling this derivative ``H560,Thy^-."
• This strain is an E. coli K-12 x E. coli C hybrid. It has K-12 restriction and modification. It is φX sensitive.
• = good miniprep dsDNA quality
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Vosberg, HP, H Hoffmann-Berling 1971. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J. Mol. Biol. 58(3):739-53.