CGSC Strain#: 5181
Strain Designation: Lin 228      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpD11(ts), pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• glpD11(ts)-- is a temperature-sensitive mutation. At 42C the cell cannot grow on glycerol as C-source and at 42C on succinate plus glycerol medium, growth stasis is observed (glycerol sensitivity).
• glpD11(ts)-- At 42C grow on Min.salts + 1%glycerol-free casein hydrolysate + 1% glucose.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant
• ts = Temperature Sensitive

References:

• Cozzarelli, NR, WB Freedberg, EC Lin 1968. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J. Mol. Biol. 31(3):371-87.