CGSC Strain#: 5065
Strain Designation: N2672      Source of Strain: Gellert & Gottesman
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, bglR7(p,op), thiE1, [lig-7](ts)
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• [lig-7](ts)-- is a temperature-sensitive mutation which was brought in from E. coli 15; it reverts. The strain must be monitored frequently.
• thiE1 was formerly called thi-1
• op = operator
• p = promoter, regulatory region
• ts = Temperature Sensitive

References:

• Gottesman, MM, ML Hicks, M Gellert 1973. Genetics and function of DNA ligase in Escherichia coli. J. Mol. Biol. 77(4):531-47.