CGSC Strain#: 4452
Strain Designation: 1000      Source of Strain: H. Hoffmann-Berling
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, rfbC1, spoT1, thiE1, creC510
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• glnX44(AS) was formerly called glnV44
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Const = constitutive

References:

• Durwald, H., H. Hoffmann-Berling 1968. Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants. J.Mol.Biol. 34:331-346