CGSC Strain#: 5062
Strain Designation: CHE-11      Source of Strain: W. Epstein
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, ptsI211, relA1, spoT1, bglR7(p,op), thiE1
Strain Comments:

• This strain is somewhat unstable and needs to be cloned before use.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• op = operator
• p = promoter, regulatory region

References:

• Gottesman, MM, ML Hicks, M Gellert 1973. Genetics and function of DNA ligase in Escherichia coli. J. Mol. Biol. 77(4):531-47.