CGSC Strain#: 5030
Strain Designation: PC1      Source of Strain: P. Carl
Sex: F- Chromosomal Markers: leuB6(Am), λ^-, thyA47, rpsL153(strR), dnaC1(ts), deoC3
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA47-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Carl, PL 1970. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol. Gen. Genet. 109(2):107-22.
• Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.
• Gross, J. 1972. Curr.Topics Microbiol.Immunol. 57:39-0