CGSC Strain#: 5022
Strain Designation: MM383      Source of Strain: M. Monk
Sex: F- Chromosomal Markers: lacZ53(Am), λ^-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2
Strain Comments:

• Although polA12 is a temperature-sensitive mutation, this strain is not temperature sensitive for growth.
• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• polA12(ts)-- : sensitive to temperature and to low salt; altered pH optimum. Normal Exo levels. Totally defective in nick translation at 42C. recA56 polA12 are ts for growth.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Monk, M, J Kinross 1972. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J. Bacteriol. 109(3):971-8.