CGSC Strain#: 7083
Strain Designation: KN321      Source of Strain: Nagata & Horiuchi
Sex: F- Chromosomal Markers: thr-33, λ^-, trpE9829(Am), serU126(ts,AS), his-2130, ligA321, tyrA15(Am), thyA707, IN(rrnD-rrnE)1, metE46
Strain Comments:

• ligA321-- was also called dnaL321.
• thyA707-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• serU126(ts,AS) was formerly called supD126
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Nagata, T, T Horiuchi 1974. An amber dna mutant of Escherichia coli K12 affecting DNA ligase. J. Mol. Biol. 87(2):369-73.
• Nagata, T, T Horiuchi 1974. An amber dna mutant of Escherichia coli K12 affecting DNA ligase. J. Mol. Biol. 87(2):369-73.