CGSC Strain#: 7096
Strain Designation: SH210      Source of Strain: Schweizer & Boos
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, Δ(argF-lac)169, zai-736::Tn10, ΔphoA8, ompF627(T2R), fadL701(T2R), relA1, glpR2(glp^c), glpD3, pitA10, spoT1, mcrB9999, creC510
Strain Comments:

• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
• glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• = tetracycline resistant
• Const = constitutive
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Schweizer, H, W Boos 1983. Transfer of the delta (argF-lac)U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion. Mol. Gen. Genet. 192(1-2):293-4.