CGSC Strain#: 4913
Strain Designation: NP37      Source of Strain: Eidlic & Neidhardt
Other Designations: BW93
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ompF627(T2R), pheS5(ts), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• This strain is malate-negative (See: Hillman and Fraenkel 1975 J. Bacteriol.122:1175)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant
• ts = Temperature Sensitive

References:

• EIDLIC, L, FC NEIDHARDT 1965. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES. J. Bacteriol. 89:706-11.
• Neidhardt, FC 1966. Roles of amino acid activating enzymes in cellular physiology. 30(4):701-19.
• Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.