CGSC Strain#: 6823
Strain Designation: DB1257      Source of Strain: D. Botstein
Sex: F- Chromosomal Markers: thrA34::Tn10, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, galK2(Oc), λ^-, tyrT58(AS), trp-89::Tn5, Rac-0, sbcB15, hisG4(Oc), rfbC1, recB21, recC22, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, hsdR0
Strain Comments:

• Related strains are described in Wyman et al.(below).
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• tyrT58(AS) was formerly called supF58
• = kanamycin resistant
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Wyman, A.R., L.B. Wolfe, D. Botstein 1985. Propagation of some human DNA sequences in bacteriophage Lambda vectors requires mutant Escherichia coli hosts. Proc.Natl.Acad.Sci.USA 82:2880-2884
• Wertman, K.F., A.R. Wyman, D. Botstein 1986. Host/vector interactions which affect the viability of recombinant phage lambda clones. Gene 49:253-262