CGSC Strain#: 4319
Strain Designation: JG85      Source of Strain: J. Gross
Sex: F' Episome/Plasmid: F197
   Plasmid Markers/Mutations: bgl-14
Chromosomal Markers: λ^-, recA56, IN(rrnD-rrnE)1, metE70, rha-2, deoC2
Strain Comments:

• This strain must be grown on medium lacking met. F197 carries the mutation bgl-14.
• recA56-- Amino acid substitution Arg 60 Cys
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)

References:

• Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.