CGSC Strain#: 6509
Strain Designation: BS21
Source of Strain: B. Sedgwick
Sex: F-
Chromosomal Markers: lon-11,
sulA1,
his-222,
thyA751,
adc-1Strain Comments: - lon-11-- is a mutation of an E. coli B gene caused by an IS186 insertion in the promoter. All derivatives of Witkin's B wildtype carry this lon mutation.
- his-222-- Not a K-12 mutation. (mutation of an E. coli B gene)
- thyA751-- Mutation in E. coli B gene
- thyA751-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
References: - Sedgwick, B., P. Robins 1980. Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response. Mol.Gen.Genet. 180:85-90
- Sedgwick, B. 1982. Genetic mapping of the ada and adc mutations affecting the adaptive response of Escherichia coli to alkylating agents. J.Bacteriol. 150:984-988