CGSC Strain#: 7510
Strain Designation: CAG12202      Source of Strain: C.A. Gross
Sex: Hfr    Point of Origin#: 44   Map Position: 46.69   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, trpC3193::Tn10kan, relA1, spoT1, thiE1
Strain Comments:

• trpC3193::Tn10kan-- Derived from trp-83::Tn10, originally characterized as trpB, but located by PCR (Nichols et al. 1998) within trpC, at nucleotide 1,317,596 (28.401 min.).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.