CGSC Strain#: 7504
Strain Designation: CAG12206      Source of Strain: C.A. Gross
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: supQ80, nadA3052::Tn10kan, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• supQ80-- mutation was found in AB259 by Russell & Pittard. Other cultures of this strain were not tested for this allele
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• = suppressor
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of