CGSC Strain#: 4244
Strain Designation: KL25      Source of Strain: M.C. Cappello
Other Designations: CSH47
Sex: Hfr    Point of Origin#: 46   Map Position: 84.96   Direction of Marker Transfer: Clockwise
Chromosomal Markers: glnV42(AS), λ^-, [LAMS]
Strain Comments:

• This is an unstable Hfr. It reverts at high frequency to F⁺. Growth is stimulated by methionine--defect uncharacterized.
• glnV42(AS) was formerly called supE42
• AS = amber(UAG) suppressor

References:

• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812