CGSC Strain#: 259
Strain Designation: Hfr 3000      Source of Strain: Pasteur Institute
Other Designations: AB259
Other Designations: Hfr H,Thi^-LAM-
Other Designations: ``HfrC"
Other Designations: JSH1
Other Designations: Hfr3000
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: supQ80, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• supQ80-- mutation was found in AB259 by Russell & Pittard. Other cultures of this strain were not tested for this allele
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• = suppressor

References:

• Bachmann, BJ 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol.Rev. 36(4):525-57.
• Theze, J., D. Margarita, G.N. Cohen, F. Borne, J.C. Patte 1974. Mapping of the structural genes of the three aspartokinases and of the two homoserine dehydrogenases of Escherichia coli K-12. J.Bacteriol. 117:133-0