CGSC Strain#: 4318
Strain Designation: KL228      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 13   Map Position: 84.98   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: leuB6(Am), lacY1 or lacZ4, glnX44(AS), gal-6, λ^-, rfbC1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812