CGSC Strain#: 4243
Strain Designation: KL96      Source of Strain: M.C. Cappello
Other Designations: AT2572
Sex: Hfr    Point of Origin#: 44   Map Position: 46.69   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812