CGSC Strain#: 4242
Strain Designation: KL99      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 42   Map Position: 23.58   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lac-42, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• This Hfr is rather unstable.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812