CGSC Strain#: 4236
Strain Designation: Hfr₆      Source of Strain: J. Lederberg
Other Designations: W3807
Sex: Hfr    Point of Origin#: 58   Map Position: 8.00   Direction of Marker Transfer: Clockwise
Chromosomal Markers: mutT2, λ^-, relA1, malA20(λ^R), spoT1, mtl-8, metB1
Strain Comments:

• This culture of Hfr₆ has acquired mutations in mal and mtl loci.
• malA20(λ^R)-- : malA operon includes malQ,P,T.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• LamR = Lambda-resistant

References:

• Hirota, Y., P.H.A. Sneath 1961. F' and F mediated transduction in Escherichia coli K12. Jpn.J.Genet. 36:307-318
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812