CGSC Strain#: 4277
Strain Designation: KL22      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: glnV42(AS), λ^-, rph-1
Strain Comments:

• This is an F- derivative of W1485E, cured by acridine orange.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• glnV42(AS) was formerly called supE42
• AS = amber(UAG) suppressor

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