CGSC Strain#: 7449
Strain Designation: CAG18452      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, tyrT666(ts,AS), zhf-3085::Tn10, rph-1, nlpA3085::Tn10, φ80
Strain Comments:

• zhf-3085::Tn10-- : This insertion was localized at 75.8, but PCR analysis by Nichols et al. (1998) located it within nlpA at nucleotide 3,837,058 (82.709 min.)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• nlpA3085::Tn10 was formerly called zhf-3085::Tn10?
• = tetracycline resistant
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Berlyn, M.K.B., K.B. Low, K.E. Rudd 1996. Linkage map of Escherichia coli K-12, Ed.9, with Appendix, Recalibrated Map Positions of Transposon Insertion Sites, M.Singer and K.B.Low. Escherichia coli and Salmonella, Neidhardt et al., ASM ASM:1715-1902