CGSC Strain#: 7099
Strain Designation: SL101/F1-10      Source of Strain: J. Speyer
Other Designations: AB1325 mud F(Tn10)
Sex: F' Episome/Plasmid: F1-10(Tn10)
   Plasmid Markers/Mutations: z--1::Tn10
   Plasmid Comment: F1::Tn10, the integration of Tn10 in F1 to give F1-10 does not interfere with transfer functions.
Chromosomal Markers: Δ(gpt-proA)62, lacY1, glnX44(AS), galK2(Oc), λ^-, hisG4(Oc), rfbC1, rpsL35(strR), xylA5, mtl-1, thiE1, rac-
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• = tetracycline resistant

References:

• Speyer, J. 1989. Pers.Commun.with B.Bachmann 9/:1989