CGSC Strain#: 7489
Strain Designation: CAG12110      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, rph-1, zjf-3186::Tn10kan
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• zjf-3186::Tn10kan-- : derived from zjf-920::Tn10, reported CoT ca. 10% with fbp and 26% with pyrB, ca.97min, but located by Nichols et al. within orf yjcS, at nucleotide 4,303,986 (95.757 min.)
• zjf-3186::Tn10kan was formerly called yjcS-3186::Tn10kan
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
• Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.