CGSC Strain#: 7480
Strain Designation: CAG18571      Source of Strain: C.A. Gross
Sex: F- Chromosomal Markers: λ^-, rph-1, cadB3182::Tn10kan
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• cadB3182::Tn10kan-- : derived from zjd-2231::Tn10, located by cotransduction at ca. 92.5, but by PCR (Nichols et al. 1998) shown to be within cadB, at nucleotide 4,356,936 (93.915 min.)
• = kanamycin resistant

References:

• Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of